Antibacterial effect of Tetrapleura Tetraptera (dawo) and Monodora Myristica (ehuru) plantextracts on Staphylococcus Aureus and Escherichia Coli
Year: 2024
Type: article
Abstract: The study was conducted to evaluate the antibacterial effect of medicinal plant extracts Tetrapleura tetraptera and Monodora myristica against specific bacteria pathogens Escherichia coli and Staphylococcus aureus. The plants were extracted using two types of extraction methods namely hot aqueous and ethanol extracts. Antibacterial testing was determined using well-in-agar diffusion method. Antibacterial activity was evaluated by measuring the inhibitory zone of the T. teraptera and M.myristica extract against the tested bacteria pathogens. Phytochemical screening was conducted on both T.tetraptera and M.myristica extracts. The results for phytochemical screening for M. myristica and T.tetraptera showed presence of alkaloids, phenols, tanins, flavoids, glycosides, saponins and terpenoids in different amounts in each of the extracts. Zone of inhibition results produced by M. myrstica , hot aquoes extract showed 11mm,4mm, at 10mg and 7.5mg concentrations against S. areus then 17mm,13mm,9mm at 10mg,7.5mg and 5.0mg against E.coli. The ethanol extract showed 15mm, 8mm,5mm at 10mg,7.5mg and 5.0mg concentrations against E. coli then 19mm, 12mm, 9mm and 4mm at all concentrations against S.aureus. Zone of inhibition results produced by T.tetraptera, hot aqueous extract showed 13mm, 8mm, 3mm at 10mg, 7.5mg and 5.0mg concentrations against E.coli then 19mm, 15mm, 1mm and 5mm at all concentrations against S.aureus. The ethanol extract showed 21mm, 11mm, 8mm, 3mm at all concentrations against E. coli then 22mm, 14mm, 12mm,5mm at all concentrations against S.aureus. Analysis of result showed that ethanol extracts were more active against the two bacteria pathogens of interest at all concentrations whereas the hot aqueous extract was more effective at 10mg, 7.5mg and 5.0mg concentrations. It also showed that the ethanol extract of T.tetraptera was most effective plant extract against the two bacteria pathogens showing higher zones of inhibition. However, when the results of the plant extracts were compared to the control used in the study which is ciprofloxacin, it had higher zones of inhibition (24mm) than that produced by the plant extracts. Due to the observed antibacterial effect of the plant extracts, they can be further studied for the development of alternative therapy to the use of antibiotic.
Author: Johnkennedy Nnodim
Institution: Rhema University
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W7128766992