Work

ATAC-seq data from DN3 and DN4 cells from WT, HEBcKO, and Rag2-KO mice

Year: 2026

Type: dataset

Abstract: Gamma delta T cells that produce IL-17 (gdT17) play essential roles in barrier immunity and autoimmunity, but the gene networks that install their functions are not well understood. We have shown that the transcriptional regulator HEB is required for efficient upregulation of Id3 in response to TCR signaling in DN3 cells, and that Id3 is essential for maturation of gdT17 cells. To evaluate how the loss of the HEB affects chromatin status in response to TCR signaling, we compared thymocyte subsets from WT and adult HEB fl/fl Vav-iCre (HEB cKO) mice, which lack HEB in all hematopoietic cells, by ATAC-seq. We sorted DN3 and DN4 thymocytes from adult WT mice and HEB cKO mice. We also sorted DN3 cells from adult Rag2-/- mice, which cannot rearrange their TCR genes and thus are unable to develop beyond the DN3 stage.

Source: DRYAD

DOI: 10.5061/dryad.4tmpg4fr5

Authors: Selvaratnam, Johanna, da Rocha, Juliana, Rajan, Vinothkumar, Wang, Helen, Reddy, Emily, Liu, Jenny, Gams, Miki, Murre, Cornelis, Wiest, David, Guidos, Cindy, Zúñiga-Pflücker, Juan Carlos, Anderson, Michele

Institutions: University of Toronto, Sunnybrook Research Institute, Hospital for Sick Children, University of California San Diego, Fox Chase Cancer Center

Language: English

Topic: T-cell and B-cell Immunology

Subfield: Immunology

Field: Immunology and Microbiology

Domain: Life Sciences

SDG: Good health and well-being

Open Access status: green

Funders: American Association of Immunologists, National Institute of Allergy and Infectious Diseases, Institute of Infection and Immunity

OpenAlex ID: W7134885650

Cites:

Cited by: 1

Related to: 10