Additional file 8: of Retrozymes are a unique family of non-autonomous retrotransposons with hammerhead ribozymes that propagate in plants through circular RNAs
Year: 2016
Type: article
Abstract: RT-PCR amplification of negative polarity retrozyme RNAs from J. curcas and F. ananassa. A: RT-PCR experiment carried out with RNA extracts from J. curcas seeds using the direct primer Jc60D for RT to make a cDNA of the minus polarity, and the adjacent primers Jc60D and Jc60fullR (left) or Jc77D and Jc77R (right) for the PCRs (see Additional file 12). B: RT-PCR experiments performed with two different RNA extracts from F. ananassa leaves using the direct primer Fa87D for RT to make a cDNA of the minus polarity, and the adjacent primers Fa87D and Fa85R for PCR (see Additional file 12). PCR products and 100 bp DNA marker were separated by native 5 % PAGEs and stained with ethidium bromide. Controls (RT-) were the same RT-PCR experiments done without adding retrotranscriptase, whereas Controls (PCR) were PCRs done without any template. Schematic representations of a full genomic retrozyme and the positions of the oligos used for RT and PCR experiments are shown below each gel picture. (PDF 649 kb)
Source: Figshare
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7136127992