〔報告〕分子生物学的手法による高松塚古墳・キトラ古墳の微生物群集構造解析
Year: 2018
Type: article
Abstract: Culture-dependent and -independent methods were applied to elucidate the microbiota involved in the biodeterioration of the 1300-year-old mural paintings, plaster walls, and stone chamber interiors of the Takamatsuzuka and Kitora Tumuli. Most isolated microorganisms were also detected as major by PCR-DGGE; however, several bacteria such as the phyla “Acidobacteria”, “Chloroflexi”, and “Cyanobacteria” were detected only via a culture-independent method (PCR-DGGE). Based on the results of this study, it was presumed that the microorganisms, such as bacteria and fungi, and mites and collembolans living in the tumulus environment invaded the stone chamber interiors of both tumuli. Moreover, it was thought that the organic acids (particularly acetic acid) produced from the microorganisms (e.g., Gluconacetobacter bacteria)not only damaged the plaster directly, but also provided nutrients to other microorganisms and enhanced their outbreaks. Major colonizers of the stone chamber interiors were assignable to ubiquitous microorganisms living in the Asuka-mura environment where both tumuli are located. To detect diverse microbiota in the tumulus samples, it was effective to use microbial community analysis using molecular methods combined with conventional isolation and cultivation methods.
DOI: 10.18953/00005724
Language: Japanese
Subfield: Earth-Surface Processes
Field: Earth and Planetary Sciences
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W7144101397