Bystander effects in non-irradiated normal cells via secreted factor(s) to medium from carbon-ion irradiated tumor cells
Year: 2018
Type: conference-paper
Abstract: It should be important for developing radiotherapy to understand the communication between irradiated tumor and non-irradiated normal cells. This year, we examined biological effects in non-irradiated normal cells, focusing on the bystander effect via secreted factor(s) to culture medium from the irradiated tumor cells. Human glioblastoma cells (T98G) were irradiated with carbon-ion microbeams generated with the TIARA in QST (LET=103keV/µm), simulating the spot scanning irradiation system. The medium from the irradiated T98G cells was added to the flask inoculated on the normal human cells, and the cells were assayed for cell-killing effect and gene mutation. The medium transfer resulted in increasing both biological effects in normal human cells. Furthermore, the induced biological effects disappeared and returned to the control levels when using the ascorbic acid. There is clear evidence that the medium from the irradiated tumor cells enable to induce damage in the neighboring non-irradiated normal cells via secreted factor(s), which were scavenged by the ascorbic acid, mediated bystander effect. (This is the co-operative study with Drs. T.Funayama, M.Suzuki and Y.Kobayashi.)
Language: Japanese
Subfield: Radiology, Nuclear Medicine and Imaging
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7145014221