正常ヒト骨芽細胞におけるbmp産生に及ぼすフェニトインの効果
Year: 1999
Type: article
Abstract: The present study was undertaken to examine the effect of phenytoin on the synthesis of bone morphogenetic proteins (BMPs). The effects of phenytoin on the gene expression of BMP-1-7 in normal human mandible-derived bone cells (HOB-M cells) were evaluated by the semiquantitative reverse transcriptionpolymerase chain reaction (RT-PCR). While phenytoin increased the mRNA levels of BMP-2 in HOB-M cells, it failed to significantly affect the mRNA levels of other BMPs. The effects of phenytoin treatment on intracellular BMP-2 synthesis in HOB-M were assessed by immunocytochemistry and the effects on the secretion of BMP-2 were evaluated by dot blotting. Phenytoin significantly increased the intensity of immunocytological staining of BMP-2 and the secretion of BMP-2. Interestingly, the stimulatory effects of phenytoin on osteocalcin secretion were completely blocked by antihuman BMP-2 monoclonal antibody. In conclusion, the present study indicates that the stimulatory effects of phenytoin on osteocalcin secretion in human bone cells are, at least, in part mediated by BMP-2.
Language: Japanese
Topic: Bone Metabolism and Diseases
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7145296288