Figure 4 from Upregulation of an Epithelial miRNA Is Associated with Immune Evasion in Progressive Bronchial Premalignant Lesions
Year: 2026
Type: other
Abstract: hsa-miR-149-5p is highly expressed within the epithelium and associates with PML progression. A, Bubble plot showing the correlation between the residual expression level of hsa-miR-149-5p and cell type marker genes, including CD3G (T cells), CD19 (B cells), CD68 (macrophages), KRT5 (basal cells), FOXJ1 (ciliated cells), MUC5AC (goblet cells), SCGB1A1 (club cells), MUC5B (secretory cells), and CEACAM5 (peri-goblet cells). Correlation results for hsa-miR-34b-5p, hsa-miR-449c-5p, and hsa-miR-150-5p are shown as controls. B, Enrichment of hsa-miR-149-5p across cell type compartments in the FANTOM5 project. Bar chart shows the normalized expression levels of hsa-miR-149-5p in the FANTOM5 samples (n = 399) (top). Vertical bars indicate the position of FANTOM5 samples derived from the epithelial (red) or immune (orange) cell compartments (bottom). C, Scatter plot of the Pearson correlation between the normalized expression levels of hsa-miR-149-5p and NLRC5 within the FANTOM5 samples derived from the epithelial cell compartment (n = 87). The dashed red line represents the linear regression fit and the shaded region indicates the 95% confidence interval. D–F, The hsa-miR-149-5p density was detected by miRNA-ISH from proliferative-subtype PMLs (n = 19; 10 progressive/persistent and nine regressive). D, Boxplot showing the hsa-miR-149-5p density per area between the epithelium and nonepithelium regions. E, hsa-miR-149-5p density per nucleus between the progressive/persistent and regressive PMLs within regions of normal epithelium, regions with hyperplasia or metaplasia (hyper-metaplasia), and regions of bronchial dysplasia. F, Representative miRNA-ISH staining of hsa-miR-149-5p for progressive/persistent PMLs (top) and regressive PMLs (bottom). Arrows indicate stained hsa-miR-149-5p within epithelium. Boxplots indicate median with IQR and whiskers indicate minimum and maximum measurement in (D) and (E). P values were FDR (Benjamini and Hochberg) adjusted and determined by Pearson correlation coefficients (A), the two-sided paired Wilcoxon test (D), and a linear mixed-effects model (E). *, P ≤ 0.05; **, P ≤ 0.01; ***, P ≤ 0.001; ****, P ≤ 0.0001; ns, no significance.
Authors: Boting Ning, Darren J. Chiu, Roxana M. Pfefferkorn, Erin Cullinane, Yohana Kefella, Erin Kane, Valerie Reyes-Ortiz, Gang Liu, Xiaohui Zhang, Hanqiao Liu, Lila Sultan, Emily Green, Myrtha Constant, Avrum E. Spira, Joshua D. Campbell, Mary E. Reid, Xaralabos Varelas, Eric J. Burks, Marc E. Lenburg, Sarah A. Mazzilli, Jennifer E. Beane
Subfield: Cancer Research
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7147635558
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