Work

Technological Aspects of Using Non-food Raw Materials From Flour Milling for Bioconversion in Ethanol

Year: 2025

Type: article

Abstract: The decisive factor in the efficiency of ethyl alcohol production from starch-containing raw materials is the depth and completeness of starch and structural carbohydrate hydrolysis into monosugars using enzyme preparations of microbial origin. The degree of utilization of starch and cellulose introduced into the technological process largely depends on the preservation of enzymatic activity, which is determined by the parameters of raw material preparation for hydrolysis, the acidity of the medium, and the applied temperature regimes. Prior to enzymatic hydrolysis, the mash was acidified to pH 5.0–5.2 with sulfuric acid, followed by liquefaction using amylolytic enzymes at a temperature of 75 °C. During the liquefaction stage, partial delignification of the cellulose-containing fraction of corn processing residues occurred as a result of acid hydrolysis, which promoted cellulose release and increased its availability for subsequent enzymatic conversion. Cellulose hydrolysis using cellulolytic enzyme preparations was carried out after the liquefaction stage at a temperature of 50 °C. Saccharification and fermentation of the mash were performed under the SSCF regime, which ensured a reduction in energy consumption due to lower liquefaction temperature, decreased enzyme dosage as a result of preserved enzymatic activity, and more complete utilization of the cellulose-containing fraction of the raw material. As a result, the concentration of fermentable sugars and the efficiency of alcoholic fermentation increased. The yield of ethyl alcohol obtained from the fermentation of corn residues under the proposed conditions reached 68 dal/t of raw material, which is 2.9 dal/t higher than that achieved under conventional liquefaction conditions at 90 °C without the use of cellulase. The obtained results can be applied in the development of energy-efficient technologies for processing starch-containing raw materials in the alcohol industry.

Source: Vibrations in engineering and technology

DOI: 10.37128/2306-8744-2025-3-15

Authors: Kateryna Danilova, Leonid Levandovskiy, Alla Solomon

Institutions: Institute of Food Resources of National Academy of Agrarian Sciences of Ukraine, Vinnytsia National Agrarian University

Language: English

Topic: Biofuel production and bioconversion

Subfield: Biomedical Engineering

Field: Engineering

Domain: Physical Sciences

SDGs: Responsible consumption and production, Affordable and clean energy

Open Access status: diamond

OpenAlex ID: W7154608593