Work

Development and Refinement of Microbial DNA Extraction Protocol from Bovine Milk

Year: 2026

Type: preprint

Abstract: Milk is a challenging matrix to extract sufficient microbial DNA from for downstream analysis. This study assessed fourteen DNA extraction protocols for their DNA outputs. An adaptation of the QIAGEN DNeasy PowerSoil kit, which increased initial sample volume and maintained all volume of lysate following bead beating proved most effective.

Source: bioRxiv (Cold Spring Harbor Laboratory)

DOI: 10.64898/2026.04.30.721090

Authors: Rowan Cook, Joana Lima, Richard J. Dewhurst, Sharon A. Huws, Christopher J. Creevey, Holly J. Ferguson

Institutions: Queen's University Belfast, Scotland's Rural College

Language: English

Topic: Milk Quality and Mastitis in Dairy Cows

Subfield: Animal Science and Zoology

Field: Agricultural and Biological Sciences

Domain: Life Sciences

SDG: Zero hunger

Open Access status: green

Funders: European Commission, Scotland’s Rural College