Análise Daexpressão Dos Receptores Toll- Like 2 E 9, E Das Vias De Sinalização Intracelular Mapkp38 E Nfkbp65 No Carcinoma Escamoso De Pênis
Year: 2019
Type: article
Study design: Observational Study
Abstract: All articles of this category (opens in new window) Modulation of the inflammatory process by TLRs may be a key factor in tumor development and progression, inducing both pro-tumor and anti-tumor responses, often through the regulation of NF-kB signaling and subsequent growth factor and anti-apoptotic proteins production. The magnitude and duration of TLR activation are critical for the development of a pro-tumor inflammatory state or anti- tumor response. To evaluate the expression of Toll-like receptors 2 and 9, and the intracellular signaling pathways NF-KBp65 and MAPKp38 in squamous cell carcinoma of the penis. 38 male patients with penile cancer, aged ≥ 18 years, were included. As a control group, 15 healthy men were included. Serum protein concentration was determined by the flow cytometry technique. For statistical analysis, the Mann-Whitney tests and Spearman’s correlation were used. It was considered significant p < 0.05. In peripheral blood, there were more leukocytes expressing TLR2 and MAPKp38 in patients, compared to healthy controls ( p = 0.002 and p < 0.0001, respectively), whereas leukocytes expressing TLR9 and NFkB were more numerous in healthy controls ( p = 0.03 and p = 0.03). In neutrophils, there was a greater number with expression of NFKBp65 and MAPKp38, in controls ( p = 0.001 and p < 0.0001). In monocytes, there were the highest number of cells expressing TLR9 and MAPKp38 in patients with penile cancer ( p = 0.003 and p = 0.01). In patients with tumors > 3.5 cm, there is a greater number of total leukocytes expressing TLR9, NFKBp65 and MAPKp38 ( p = 0.01, p = 0.008 and p = 0.03, respectively), a higher amount of neutrophils with expression of TLR2 and NFKBp65 ( p = 0.0008 and p = 0.01).In tumors ≤ 3.5 cm, a higher amount of monocytes expressed NFKBp65 ( p = 0.02).There was a higher number of monocytes and neutrophils with TLR9 expression ( p = 0.009 and p = 0.0003) . In tumor tissue, there was a greater expression of TLR9 and NFKBp65 in patients with tumors ≤ 3.5 cm ( p = 0.02).In the tumor tissue, there was a moderate positive correlation between TLR2 and TLR9 (r = 0.65, p = 0.0009) and TLR9 and NFKBp65 (r = 0.60; p = 0.003), and a high positive correlation between TLR2 and NFKBp65 (r = 0.78; p < 0.0001). There are changes in blood levels of TLR2, TLR9, NFKBp65 and MAPKp38, at different levels, depending on the cell type, and on tumor tissue that may function as prognostic and predictive factors of treatment for patients with penile SCC. Publication History Article published online: 23 October 2019 © 2019. The Author(s). This is an open access article published by Thieme under the terms of the Creative Commons Attribution 4.0 International License, permitting copying and reproduction so long as the original work is given appropriate credit (https://creativecommons.org/licenses/by/4.0/) Thieme Revinter Publicações Ltda. Rua do Matoso 170, Rio de Janeiro, RJ, CEP 20270-135, Brazil Bibliographical Record Felipe da Silva Marinho, Felipe Dubourcq Barros, Mauro Marques, Leuridan Torres, Fernando Augusto Soares. ANÁLISE DAEXPRESSÃO DOS RECEPTORES TOLL- LIKE 2 E 9, E DAS VIAS DE SINALIZAÇÃO INTRACELULAR MAPKP38 E NFKBP65 NO CARCINOMA ESCAMOSO DE PÊNIS. Brazilian Journal of Oncology 2019; 15. DOI: 10.1055/s-0044-1797685
Source: Brazilian Journal of Oncology
Institution: Hospital de Câncer de Pernambuco
Language: English
Topic: Genital Health and Disease
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7162799208