Preclinical Assessment of the Wound Healing Efficacy of Hemidesmus indicus Hydroalcoholic Root Extract on Cyprinus rubrofuscus
Year: 2026
Type: article
Abstract: Background: Effectively managing wounds presents a significant challenge, as skin injuries frequently result in secondary bacterial infections and the limitations of existing treatments, including high cost and adverse reactions. As a result, there has been a growing interest in using herbal remedies. To improve animal welfare and experimental effectiveness, Hemidesmus indicus, which is well-known for its folklore and its antibacterial and antioxidant qualities, was evaluated for its capacity to heal wounds using a fish model. Aim: This study aims to explore the potential wound healing properties of H. indicus root hydroalcoholic extract on Cyprinus rubrofuscus. Methods: After anaesthesia, standardised excision wounds or burn wounds were created in the region between the pectoral fin and dorsal fin of each fish (C. rubrofuscus) in all experimental groups. Group I served as control, Group II (standard) received providone iodine solution (0.1%), Group III (Test I) received a test root extract of H. indicus (25 mg/L), and Group IV (Test II) received a test root extract of H. indicus (10mg/L). The healing progression was evaluated by noting the time for complete epithelialisation; 50% wound contraction, and histopathological analysis was also performed. These experimental findings revealed that animals treated with H. indicus root hydroalcoholic extract exhibited a significantly faster wound contraction rate compared to the control group in both models, in a dose-dependent manner. Result: In the excision wound model, complete wound contraction was observed within 15.833±0.401 days at 25 mg/L and 17.5±0.718 days at 10 mg/L, whereas in the burn wound model, complete wound contraction occurred within 18.333±0.666 days at 25 mg/L and 19.833±0.6 days at 10 mg/L. Both treatments showed statistically significant improvement compared to the control group (p < 0.01). Conclusion: This research indicates that the hydroalcoholic extract from the roots of H. indicus has strong wound-healing capabilities. Major Findings: The hydroalcoholic root extract of H. indicus showed dose-dependent wound healing in excision and burn models on C. rubrofuscus. These findings highlight its strong wound-healing potential and support its traditional use.
Source: Journal of Natural Remedies
Institution: Seacom Skills University
Language: English
Topic: Wound Healing and Treatments
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7167478917