Year: 2026
Type: article
Abstract: The aim. To update approaches to the standardization of Angelica archangelica rhizomes and roots, taking into account modern requirements, during a comprehensive study. Materials and Methods. Morphological and anatomical features of Angelica archangelica rhizomes and roots were studied using light microscopy. Luminescence of tissues and a working standard sample of the coumarins eleutheroside B1 (7-O-glucoside of isofraxidine) were studied by luminescent microscopy, and vascular structures were studied by polarization microscopy. The profile of volatile compounds of lipophilic fractions of water-alcohol extract and essential oil was determined by gas chromatography with mass spectrometric detection (GC-MS). The total coumarins content was determined by UV spectrophotometry. Results . Rhizomes and roots of Angelica archangelica of Altai origin were studied for the first time. The most significant results were obtained at the excitation wavelengths in the range of 330 to 400 nm — light blue luminescence of phloem cells and secretion of schizogenous cavities was observed, due to substances of a coumarin nature. The expediency of using a histochemical reaction with Sudan III solution, which reveals schizogenous cavities, secretion droplets, and cell walls of vascular elements, has been substantiated. For the diagnosis of specific conducting elements in Angelica raw material (powder), the use of polarization microscopy is recommended. Using GC-MS for lipophilic fractions of water-alcohol extract and essential oil, a chromatographic profile was established, including substances of both terpene and coumarin nature: prenylated coumarin osthol (up to 49.72 %) and epilobulol (25.61 %). The total coumarin content in the studied raw material was 1.50 ± 0.08 % calculated as coumarin. Conclusion . A comprehensive approach to the analysis of Angelica archangelica rhizomes and roots, involving the use of both widely used (macroscopic, microscopic, spectrophotometric) and limited-use (luminescent, polarization microscopy, and GC-MS) methods, forms the basis for the development of a draft new pharmacopoeial article “ Angelica archangelica Rhizomes and Roots.”
Source: Pharmacy & Pharmacology
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
Funder: University of Dhaka
OpenAlex ID: W7170201625