Work

Gomisin G Inhibits Pancreatic Cancer Cell Growth by Promoting YAP Degradation

Year: 2026

Type: article

Abstract: , is known for its anti-tumor, anti-inflammatory, and antioxidant properties. However, its specific effects on pancreatic cancer and the underlying mechanisms remain unclear. In this study, we used a colony formation assay to evaluate the impact of Gomisin G on pancreatic cancer cell proliferation and colony formation. Flow cytometry and western blot analyses revealed that Gomisin G induces G1 phase arrest by modulating the expression of cyclin D1, p21, p27, and Rb activity, and triggers apoptosis in pancreatic cancer cells. Using a mouse xenograft model, we found that Gomisin G significantly inhibits the growth of PANC-1 pancreatic tumors. Mechanistically, RNA-seq, western blot, qPCR, and confocal microscopy showed that Gomisin G promotes LATS1-mediated YAP phosphorylation, accelerates YAP protein degradation, reduces its nuclear localization, and down-regulates the mRNA levels of the target genes CTGF and CYR61 in the Hippo-YAP pathway. Overall, our findings suggest that Gomisin G has significant anti-cancer activity against pancreatic cancer and may be a promising candidate for therapeutic development.

Source: Food Science & Nutrition

DOI: 10.1002/fsn3.72111