Work

Isolation and molecular identification of klebsiella spp. from poultry and agricultural settings: A morphological and genotypic approach

Year: 2026

Type: article

Abstract: Klebsiella is an emerging pathogen of One Health importance. Klebsiella species comprises of normal intestinal flora of poultry, but could be opportunistic whenever the immune system of affected bird is compromised. The increasing emergence of antimicrobial-resistant Klebsiella strains in poultry presents significant concerns for animal health, food safety, and public health due to the zoonotic potential of these bacteria. This study aims to investigate the prevalence, of Klebsiella spp. in poultry, with a focus on their isolation from gastrointestinal, respiratory, and farm environmental samples. Both phenotypic and genotypic methods were employed for identification, including selective culturing, biochemical profiling, and molecular confirmation using PCR. One hundred fifty samples were collected for bacteriological isolation and confirmation of Klebsiella and K. pneumoniae by phenotypic and genotypic methods. Klebsiella spp were isolated from 17 (11.3%) out of 150 samples. The prevalence of K. pneumoniae in poultry birds and farm environment was 8.0%.

Source: International Journal of Advanced Biochemistry Research

DOI: 10.33545/26174693.2026.v10.i7m.9292