Antimicrobial resistance in bacteria isolated from environmental surfaces in a municipal dog shelter: a One Health approach
Year: 2026
Type: article
Abstract: Shared environments can act as reservoirs of resistant bacteria, facilitating their dissemination between species and posing a risk to public health. Dog shelters, often characterized by high animal density and limited sanitary infrastructure, may favor the persistence and circulation of these microorganisms. This study aimed to evaluate the antimicrobial resistance profile of bacteria present on environmental surfaces in a municipal dog shelter located in northwestern Paraná, Brazil. Sixty samples were collected from frequently handled surfaces using sterile swabs. The samples were cultured in Brain Heart Infusion broth and subsequently plated on blood agar for the isolation of Staphylococcus spp. and Enterobacteriaceae. Identification was performed based on morphological characteristics and biochemical tests, while antimicrobial resistance was determined using the disk diffusion method. Isolates resistant to oxacillin and cefoxitin were subjected to PCR for the detection of the mecA gene. Among the samples analyzed, 45 % were identified as Staphylococcus spp. and 11 % as enterobacteria. Most staphylococcal isolates showed high resistance to several antimicrobials, with 88.9 % classified as multidrug-resistant. None of the isolates carried the mecA gene. These results demonstrate the presence of multidrug-resistant bacteria on shelter surfaces, indicating a potential risk of dissemination among animals, humans, and the environment.
Source: Ciência Animal Brasileira
Authors: Willian Fontini Marangon, Francieli Gesleine Capote Bonato, Halison Murilo da Silva Oliveira, Kariny Aparecida Jardim Rúbio Espessato, Jorge Fernandes de Azevedo, Henrique Susumu Tanaka, Isabela Carvalho dos Santos, Heloiza de Oliveira Marchi, Vitor Yukio Takahashi, Regiane Pereira Baptista da Silva, Lidiane Nunes Barbosa, Daniela Dib Gonçalves
Institution: Universidade Paranaense
Language: English
Subfield: Infectious Diseases
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7202009088