CHO-K1 cell-derived scFv targeting pseudorabies virus ameliorates multi-organ viremia and improves animal survival in a lethal infection model
Year: 2026
Type: article
Abstract: The pseudorabies virus, with enhanced virulence, increased transmissibility, and severe co-infections, have re-emerged worldwide, leading to significant economic losses in swine industry. However, the traditional Bartha-K61 vaccine could not provide full protection against the PRV variant strains, thus, an urgent need for the development of novel prophylactic and therapeutic strategies. Here, we proposed a novel antibody construct, PRV single chain fragment variable (PRV-scFv), for PRV antibody therapy. The PRV-scFv genes were constructed by joining the PRV-VH and PRV-VL sequences, isolated from the immunized mice’s spleen, via a flexible (Gly4Ser)3 linker. This construct was stably expressed in CHO-K1 cells cultured in serum-free suspension. Based on these findings, we screened a monoclonal cell line, CHO-K1-PRV-scFv-2 E8 cell, to robustly express PRV-scFv antibody that effectively inhibited PRV infection in vitro , reduced the viral load in vivo , and improved the survival rate of infected mice, which provided new insights into antibody therapy and veterinary therapeutic strategies development.
Source: Frontiers in Microbiology
Institutions: Northwest Minzu University, Gansu Agricultural University
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,801
OpenAlex ID: W7202071905