Work

Investigating angiotensin II concentration in modulating Plasmodium falciparum infection and angiotensin receptor activation

Year: 2026

Type: article

Abstract: Introduction Angiotensin II (Ang II) has been proposed to protect from severe malaria by killing the parasite and maintaining the blood-brain barrier integrity by acting through type 1 (AT 1 R) and type 2 (AT 2 R) receptors. However, this protection from severe malaria is counterproductive in adulthood as the elevated plasma Ang II puts the individual at risk for developing hypertension. Various natural and synthetic peptides of Ang II have been explored to check its antiplasmodial actions despite Ang II being a significant vasopressor via its actions through AT 1 R. Therefore, to be able to explore Ang II as a potential antiplasmodial agent, it is important to demonstrate its IC 50 against P. falciparum in vitro and its minimum effective concentration required for activation of AT 1 R. It is desirable that IC 50 of Ang II against P. falciparum remains below the threshold for AT 1 R activation and hence to verify this, the current study was planned as there are no previously published reports for determining the threshold Ang II concentration for AT 1 R activation. Methods For invasion inhibition assay, Pf-infected RBCs (schizonts) were incubated with four different concentrations of Ang II for 16 hours. The activation of AT 1 R and AT 2 R in the presence of Ang II (from 10 –9 M to 10 –7 M) was tested by calcium and cGMP signaling assays, respectively using the human brain microvascular endothelial cell line as an in vitro model. Results Effective Ang II concentration among all tested concentrations was found to be 10 –6 M against Plasmodium falciparum invasion. Statistically non-significant and negligible responses in AT 1 R and AT 2 R activation were observed below the desired Ang II concentration of 10 –6 M. Discussion The results indicate that the IC 50 of Ang II against P. falciparum is below the threshold for AT 1 R activation. However, the minimum effective Ang II concentration required for AT 1 R activation remains unanswered which demands further improvement in experimental designs.

Source: Frontiers in Parasitology

DOI: 10.3389/fpara.2026.1852476

Authors: Auley De, Aparna Tiwari, Medha Agarwal, Veena Pande, Abhinav Sinha

Institutions: Indian Council of Medical Research, National Institute of Malaria Research, Kumaun University, Academy of Scientific and Innovative Research

Language: English

Topic: Malaria Research and Control

Subfield: Public Health, Environmental and Occupational Health

Field: Medicine

Domain: Health Sciences

SDG: Good health and well-being

Open Access status: gold

Estimated APC paid: $2,353