Figure 4 from An Integrated Spatial Multi-Omics Workflow for Sequential RNA and Protein Profiling in FFPE Tumor Tissue
Year: 2026
Type: other
Abstract: β-Catenin transcript and protein expression patterns identify Wnt signaling signatures in tumor cells. A–C, Representative coregistered Xenium and IMC images of two tumor samples and an adjacent normal liver tissue. A, Tumor sample containing a tertiary lymphoid structure adjacent to tumor cells expressing keratin 8/18 and demonstrating nuclear and cytoplasmic localization of β-catenin protein and expression of CTNNB1 transcript. B, Tumor sample with epithelial-like tumor cells expressing intermediate levels of keratin 8/18 with cytoplasmic and nuclear β-catenin protein expression and lower expression of CTNNB1 transcript. C, Tumor-adjacent normal liver tissue with minimal expression of β-catenin protein and CTNNB1 transcript. The β-catenin protein and CTNNB1 transcript heatmaps were generated using CytoMap. D, Heatmaps showing the raw transcript counts of epithelial markers (EPCAM and CDH1), stemness-associated genes (SOX9 and LGR5), and EMT-associated gene SNAI2 in tumors 1 and 2, revealing signatures associated with EMT in tumor 1. The gene expression heatmaps for epithelial markers, stemness markers, and SNAI2 were generated using ImageFeaturePlot function from Seurat (v5.3.0; ref. 46). Scale bars, 200 μm.
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: gold
OpenAlex ID: W7203718490