Pathogenicity of Pseudomonas syringae DC3000 on green beans and identification of resistance sources
Year: 2024
Type: article
Abstract: The study aimed to investigate the pathogenicity of the model bacterium Pseudomonas syringae pv. tomato (Pst) DC3000 in relation to Fabaceae crops and to isolate sources of resistance of green beans to Pst DC3000. The material for the study was 24 varieties of green beans, one variety of broad beans and one variety of green peas. To study the virulence of the analysed bacterium in relation to legume crops, five variants of infection at different stages of plant ontogenesis were used – sprouts, formation of cotyledons, primordial and the first pair of true leaves. According to the results of the analysis of variance, a significant effect of both the infection method and varietal specificity on the outcome of infection was observed: 63.6% of variance for the inoculation method, 29.8% of variance for the variety and 6.6% of variance for their interaction. The most sensitive stage of ontogenesis for plant infection is the primordial leaf phase, at which the damage index of the analysed bean varieties was 0.5-4 points. When inoculating bean and pea varieties with Pst DC3000, the average damage index of sprouts and seedlings was at the level of 0-0.5 points and did not statistically differ from that of the plants in the control group. The correlation coefficient between the manifestation of wilting and chlorosis of leaves during the first and second counts was close and reliable (p<0.005) – r=0.95; Sr=0.06; tr 16.38 > ttheor. 2.01). When assessing 22 collection samples of green beans for resistance to Pst DC3000, the damage index on the 14th day varied from 0 to 4 points. Five varieties of domestic and foreign selection had high resistance to the Pst DC3000 bacteria: Supernano, Minidor, Alonso, Rosinka, Nadezhda, for which the degree of development (R) and the degree of spread of the disease (P) were at the level of 0-12%.
Language: English
Subfield: Plant Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: hybrid
OpenAlex ID: W7203791990