Year: 2026
Type: preprint
Abstract: This microsatellite genotyping preparation method is used in our lab for routine genotyping analysis of beluga whale (Delphinapterus leucas) biopsy samples. It involves the use of 18 primer pairs for the amplification of 18 nuclear microsatellite loci, 17 of which are informative polymorphic loci and one* of which is used as a "peacemaker" locus to ensure genotyping accuracy. Loci are amplified across six multiplex reactions, which have been optimized for performance in our lab. The loci used are: EV14 (Valsecchi & Amos, 1996) EV37Mn (Valsecchi & Amos, 1996) EV94 (Valsecchi & Amos, 1996) FCB1 (Buchanan et al., 1996) FCB3 (Buchanan et al., 1996) FCB4 (Buchanan et al., 1996) FCB5 (Buchanan et al., 1996) FCB6 (Buchanan et al., 1996) FCB10 (Buchanan et al., 1996) FCB14 (Buchanan et al., 1996) FCB17 (Buchanan et al., 1996) MK6 (Krützen et al., 2001) RW34 (Waldick et al., 1999) RW48 (Waldick et al., 1999) SW19 (Richard et al., 1996) TexVet5 (Rooney et al., 1999) TexVet19 (Rooney et al., 1999) *GATA028 (Palsbøll et al., 1997) Detailed primer sequences can be found in the Materials section.
Authors: Timothy Frasier, Brenna Frasier
Institutions: Nova Scotia Museum, Saint Mary's University
Language: English
Subfield: Genetics
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7203837596