Administration of extract ethanol of Lime peel (Citrus aurantiifolia) effective as anti-hyperlipidaemia: In vivo study
Year: 2026
Type: article
Abstract: The quest for natural alternatives to existing pharmaceutical therapies for hyperlipidaemia has been prompted by the known harmful effects of these medications, which are a major risk factor for cardiovascular disease. Lime (Citrus aurantiifolia) peel ethanol extract was tested for its antihyperlipidemic effects on total cholesterol and triglyceride levels in mice that had been hyperlipidaemic due to a high-fat diet. In a genuine experimental study with a pre- and post-test control group design, twenty-five male Mus musculus (ddy strain), two to three months old, weighing twenty to thirty grams each, were split into five groups: one served as a negative control, another as a positive control (simvastatin 0.10 mg/20g BW), and the three treatment groups that received lime peel extract (1, 2, and 2.8 mg/20g BW) were also given a weight. The 96% ethanol maceration process yielded 1.02% of the final extract. Boiling duck egg yolk at a 20% concentration for 14 days caused hyperlipidaemia. There was confirmation of flavonoids by the Shinoda test. Enzymatic colorimetric techniques were used to evaluate lipid levels on both Day 0 and Day 7. Two-Way ANOVA and Tukey's HSD were used for data analysis (p<0.05). Flavonoids were validated by phytochemical screening. Lipid reductions were statistically substantial (p<0.001) across all treatment groups. Similar to simvastatin, the most significant decrease was shown in triglycerides (from 212.8±24.6 to 73.8±5.0 mg/dL) and cholesterol (from 275.2±24.9 to 90.6±5.4 mg/dL) at the optimum dose of 2.8 mg/20g BW. Treatment, time, and their interaction were shown to have significant impacts according to Two-Way ANOVA (p<0.001). With 2.8 mg/20g BW showing excellent antihyperlipidemic action, an ethanol extract of Citrus aurantiifolia peel successfully reduces total cholesterol and triglyceride levels in hyperlipidaemic mice, indicating its promise as a natural treatment option.
Source: Jurnal Teknologi Laboratorium
Institutions: Universitas Noor Huda Mustofa, Universitas Borneo Lestari
Language: English
Subfield: Pharmacology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W7203860025