Effects of chronic single – and dual–band radiofrequency exposure on spatial learning and memory retention in growing male rats
Year: 2026
Type: article
Abstract: Radiofrequency electromagnetic fields have become increasingly present in daily life due to the widespread use of wireless technologies. However, their long–term effects on cognitive functions remain insufficiently understood, particularly under repeated multi–frequency exposure conditions. This study investigated the effects of chronic exposure to 800 MHz, 900 MHz, and sequential 800/900 MHz RF fields on spatial learning and memory in growing male Wistar rats. Animals were divided into four groups: sham–exposed, 800 MHz, 900 MHz, and sequential 800/900 MHz exposure. Exposures were administered 6 days per week for 4 months. Spatial learning and memory were assessed using the Morris water maze. During the acquisition phase, rats underwent four trials per day for five consecutive days, and escape latency was recorded. Memory retention was evaluated during the probe trial by measuring time spent in the target quadrant and platform–site crossings. Escape latency significantly decreased across training days in all groups, indicating preserved acquisition learning. No significant effect of exposure was observed during the acquisition phase. In contrast, probe trial analysis revealed a significant reduction in target–quadrant time in the sequential 800/900 MHz group compared with sham–exposed and 800 MHz animals, whereas platform–site crossings were not significantly affected. Overall, chronic radiofrequency exposure did not impair spatial learning acquisition, but sequential exposure to 800 and 900 MHz was associated with reduced spatial memory retention.
Language: English
Subfield: Biophysics
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W7204192377