Evidence of the Effect of opcA on the Catalytic Activity and Redox Sensitivity of Glucose-6-Phosphate Dehydrogenase of Synechocystis sp. PCC6803
Year: 2026
Type: article
Abstract: This study aimed at the analyses of G6PDH activity under presence of reducing agent DTTred using cell-free extracts of opcA− mutant Synechocystis sp. PCC6803 cells. Km values of enzyme for both G6P and NADP was higher in cell-free extracts of the opcA− mutant in comparison with those of the wild type showing that the OpcA polypeptide plays a role to increase the substrate affinity of G6PDH. The enzyme was insensitive to DTTred in opcA− mutant cell-free extract at pH 7.5 while the activity reduced about 29.31% in wild type cell-free extracts indicating that presence of the OpcA was necessary to alter redox state of the enzyme by DTTred. Results of this study support that the OpcA protein, as one of the components of a cellular level regulation, plays an important role in redox modulation of cyanobacterial G6PDH.
Institution: Ondokuz Mayıs University
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W7204215063