EFFECT OF ORANGE (Citrus sinensis) PEEL POWDER SUPPLEMENTATION ON GROWTH, SURVIVAL AND IMMUNE RESPONSE AGAINST Aeromonas hydrophila IN ROHU (Labeo rohita)
Year: 2026
Type: article
Abstract: The present study aimed to evaluate the effect of orange (Citrus sinensis) peel powder supplementationon growth, survival and immune response against Aeromonas hydrophila in Rohu (Labeo rohita) at theDepartment of Aquaculture, College of Fisheries Science, Kamdhenu University, Veraval. Theexperiment included five treatments; a control group and four experimental groups supplemented with0.25%, 0.50%, 0.75% and 1.00% orange peel powder (OPP), respectively. The experimental design wascompletely randomized design with four replications. The mean weight, mean weight gain, FCR, SGR,PER, FCE and survival of the experimental fish (Labeo rohita) were recorded fortnightly throughout theexperimental period. The results of the present study showed that the highest weight was found in 1.00%orange peel powder (5.10 ± 0.03 g), followed by 0.75% (4.73 ± 0.03 g), 0.50% (4.50 ± 0.05 g), 0.25%(4.16 ± 0.04 g) and the control group (T0) (3.75 ± 0.05 g). The SGR, FCE, FCR, PER and survival ratewere also higher in the 1.00% OPP supplemented group. To determine how the diets affected theimmune system, total erythrocyte count (TEC), total leucocyte count (TLC), nitroblue tetrazolium test(NBT), lysozyme activity and myeloperoxidase activity (MPO) was measured and 1 % OPP is effective.Disease resistance to Aeromonas hydrophila was assessed through a challenge test after 60 days of theexperimental period, revealing that the 1.00% OPP had a higher survival rate than all other treatments aswell as the control group. In conclusion, the findings of this study demonstrate that 1.00%supplementation of orange peel powder in the diet of Rohu fish (Labeo rohita) positively impacts theirgrowth, feed utilization, immune parameters and disease resistance.
Source: PLANT ARCHIVES
Institution: Kamdhenu University
Language: English
Subfield: Aquatic Science
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W7204230104