Optimization of Crude Polysaccharide Yield From Brassica rapa L. Using Ultrasound‐Assisted Deep Eutectic Solvents: A Comparative Study of Antioxidant Activity and Content From Different Regions
Year: 2026
Type: article
Abstract: This study optimized the extraction of crude polysaccharide fractions from turnip ( Brassica rapa L.) using ultrasound‐assisted deep eutectic solvents (UAE–DES) and compared the crude polysaccharide yields and antioxidant activities of turnip samples collected from three geographic regions of China. Four DES systems were evaluated, and choline chloride–urea (1:2) was identified as the most effective solvent. Extraction parameters were further optimized by single‐factor experiments combined with response surface methodology, yielding optimal conditions of 64°C, 20% water content, and 46 min ultrasonic time. Under these conditions, the crude polysaccharide yield reached 11.1%. The highest crude polysaccharide yields were obtained from the Xinjiang Keping sample, followed by those from Xinjiang Urumqi and Shanxi Yuncheng. Antioxidant activities, assessed by DPPH and ABTS + radical scavenging assays, showed regional variation, with Xinjiang Keping samples exhibiting the highest ABTS + scavenging activity (91.8%). These findings demonstrate that UAE–DES is an effective and environmentally friendly approach for extracting crude polysaccharide fractions from turnip. Furthermore, this study provides preliminary evidence that regional samples differ in crude polysaccharide yield and antioxidant activity, providing a basis for further investigation among geographic origin, polysaccharide composition, and biological activity.
Source: Journal of Chemistry
DOI: 10.1155/joch/8967563
Institutions: Yuxi Normal University, Kunming Medical University
Language: English
Subfield: Food Science
Domain: Life Sciences
SDG: Zero hunger
Open Access status: gold
Estimated APC paid: $2,160
Awards: G2013966708, G3960163050
OpenAlex ID: W7204252811