Cross-Regional Reanalysis of 16S rDNA Amplicon Sequence Data Reveals Core Taxa and Regional Modular Dynamics in the Panax ginseng Rhizosphere Bacterial Community
Year: 2026
Type: article
Abstract: The rhizosphere microbiome plays a crucial role in the development, health, and therapeutic properties of Panax ginseng. Therefore, we evaluated the spatial trends in bacterial communities using a comprehensive reanalysis of 359 sequenced rhizosphere samples from seven NCBI Sequence Read Archive BioProjects. The SILVA reference database was used to compare the Chinese and Korean sequencing datasets, focusing on quality filtering, denoising, amplicon sequence variants, and taxonomy assignment. The bacterial community structure was characterized, and differentially abundant taxa were identified using the Kruskal–Wallis test with false discovery rate correction. In addition, based on the detected bacterial taxa, potential ecological traits were contextually interpreted to provide insight into their potential ecological significance. Despite regional and technical variations, 37 bacterial taxa consistently emerged as conserved core microbiome members. Regional analyses revealed distinct bacterial drivers, such as Ligilactobacillus, Romboutsia, Nitrospira, and Bradyrhizobium in the Korean samples and Holophaga and Arachidicoccus in the Chinese samples. Putative functional annotations showed that plant-associated defense mechanisms, nutrient mobilization, and carbon cycling varied geographically. Overall, these findings indicate that although a conserved core community exists, regional differences may influence the composition, functional potential, and ecological relationships of the P. ginseng rhizosphere.
Source: Plants
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: gold
Estimated APC paid: $3,258
OpenAlex ID: W7204456872