Work

Green fabrication of Asparagus racemosus extract-loaded metallic nanoparticles: In-Vitro antioxidant and anticancer evaluation

Year: 2026

Type: article

Abstract: Objective: Asparagus racemosus is a medicinal plant containing diverse phytoconstituents, including glycosides, alkaloids, steroids, tannins, lignans, and other biologically active constituents. The present investigation was undertaken to improve the performance of poorly water-soluble constituents present in the chloroform extract through green synthesis of metallic nanoparticles. Materials and Methods: The plant material was extracted by Soxhlet extraction following defatting with petroleum ether, and potentially active constituents were investigated Nanoparticles were prepared using different concentrations of the metal salt and were characterized by UV-visible spectroscopy, particle-size and zeta-potential measurements, ESEM, TEM, FTIR, and thermal analysis. Results: The optimized formulation was further evaluated for antioxidant activity by the DPPH assay and anticancer activity by the MTT assay. Formulation SCCN4 showed a particle size of 276.2±8.1 nm, zeta potential of -50.21±7.5 mV, and PDI of 0.29. Microscopic examination showed predominantly spherical particles with a concentric bilayer appearance. FTIR analysis indicated band shifts associated with nanoparticle formation without evidence of major chemical incompatibility. TGA and DTA findings indicated satisfactory thermal stability. The optimized nanoparticles produced greater DPPH radical-scavenging activity than the extract and demonstrated activity against MCF-7 and LNCaP cells. Conclusion: Overall, SCCN4 showed improved in-vitro performance compared with the parent extract, supporting further investigation of the nanoparticle system.

Source: International Journal of Pharmaceutical Research and Development

DOI: 10.33545/26646862.2026.v8.i8b.437