Year: 2026
Type: article
Study design: Observational Study
Abstract: OBJECTIVE: To investigate plasma miR-1294 levels in cholangiocarcinoma (CCA) patients compared with healthy individuals and Opisthorchis viverrini-infected subjects. METHODS: To assess its potential as a plasma-based diagnostic biomarker, plasma samples were collected and total RNA was extracted from 15 healthy controls (HC), 12 O. viverrini (OV)-infected individuals, and 33 patients with CCA. miR-1294 expression was quantified using RT-qPCR (MiRXES). RESULTS: Relative expression analysis demonstrated a significant upregulation of plasma miR-1294 in patients with CCA compared with both HC and OV groups (P < 0.0001). Receiver operating characteristic (ROC) curve analysis showed that miR-1294 discriminated CCA from non-CCA subjects, yielding an area under the curve (AUC) of 0.8676, with a specificity of 96.30% and a sensitivity of 63.64%. CONCLUSION: The upregulation of plasma miR-1294 was investigated in this study. Despite these promising findings, the moderate sensitivity suggests that miR-1294 alone may not be sufficient as a standalone diagnostic marker. Nevertheless, its high specificity and non-invasive detectability highlight its potential utility as a complementary biomarker, particularly in combination with other diagnostic indicators for improved detection of CCA.
Language: English
Subfield: Surgery
Field: Medicine
Domain: Health Sciences
Open Access status: gold
Estimated APC paid: $200
Funder: Thammasat University
Award: G6126532520
OpenAlex ID: W7204536574