Establishment of in vitro aseptic culture of the rare species Cercis occidentalisBoiss. in Ukraine
Year: 2025
Type: conference-paper
Abstract: The optimisation of existing landscapes and the construction of new ones has long been carried out with the use of valuable ornamental woody plants. The source of increasing their range was and still is mobilisation, propagation, conservation and widespread introduction into the initial trial with further use in the urban phytoptimisation system. The presence of plants with high decorative properties is of particular value, in particular for landscaping populated areas. The representative of this group is a species of Cercis occidentalis Voss. from the family Fabaceae Lindl., which is rare in Ukraine, whose decorative properties are most evident during the flowering period, when even before the leaves appear, a mass of crimson-lilac flowers appears on the trees, collected in inflorescences of 8-16 pieces. The appearance of green, glossy, heart-shaped leaves against the background of a flowering tree and the formation of a tent-like crown shape further enhances its decorative effect. Obtaining planting material for these plants often requires the use of vegetative propagation, as traditional methods are often either difficult to perform or not available at all. Therefore, it is in vitro technologies that have been very successful in producing large quantities of genetically homogeneous, virus-free planting material in a short time with a minimum number of plants of the source material. This is one of the most modern, high-tech and promising methods of plant propagation. Successful establishment of in vitro aseptic culture propagation and preservation of plants in in vitro culture can be achieved by observing the correct selection of plant material, surface decontamination, composition of culture media, optimisation of cultivation conditions, which ensures the growth and development of plants introduced in vitro. Therefore, the aim of our research was to find out the peculiarities of introducing C. occidentalis plant material into in vitro culture and obtaining sterile, viable explants for further cultivation on nutrient media containing phytohormones. Young green shoots of C. occidentalis with apical and lateral buds, from which microscopic shoots 0.8-10.0 mm long were isolated to obtain explants, The optimisation of existing landscapes and the construction of new ones has long been carried out with the use of valuable ornamental woody plants. The source of increasing their range was and still is mobilisation, propagation, conservation and widespread introduction into the initial trial with further use in the urban phytoptimisation system. The presence of plants with high decorative properties is of particular value, in particular for landscaping populated areas. The representative of this group is a species of Cercis occidentalis Voss. from the family Fabaceae Lindl., which is rare in Ukraine, whose decorative properties are most evident during the flowering period, when even before the leaves appear, a mass of crimson-lilac flowers appears on the trees, collected in inflorescences of 8-16 pieces. The appearance of green, glossy, heart-shaped leaves against the background of a flowering tree and the formation of a tent-like crown shape further enhances its decorative effect. Obtaining planting material for these plants often requires the use of vegetative propagation, as traditional methods are often either difficult to perform or not available at all. Therefore, it is in vitro technologies that have been very successful in producing large quantities of genetically homogeneous, virus-free planting material in a short time with a minimum number of plants of the source material. This is one of the most modern, high-tech and promising methods of plant propagation. Successful establishment of in vitro aseptic culture propagation and preservation of plants in in vitro culture can be achieved by observing the correct selection of plant material, surface decontamination, composition of culture media, optimisation of cultivation conditions, which ensures the growth and development of plants introduced in vitro. Therefore, the aim of our research was to find out the peculiarities of introducing C. occidentalis plant material into in vitro culture and obtaining sterile, viable explants for further cultivation on nutrient media containing phytohormones. Young green shoots of C. occidentalis with apical and lateral buds, from which microscopic shoots 0.8-10.0 mm long were isolated to obtain explants,
Authors: Larysa Koldar, Liubovi Isciuc
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W7204701424