Phytochemical Profiling and Antioxidant Activity of Selected Fruit Crops Using Advanced Analytical Techniques
Year: 2026
Type: article
Abstract: The growing recognition of fruits as rich sources of bioactive phytochemicals has intensified the need for comprehensive analytical strategies to profile their diverse secondary metabolites and accurately assess antioxidant capacity.Traditional techniques like HPLC-DAD and UV-Vis spectrophotometry have a number of disadvantages, including lack of structural resolution, low sensitivity for trace compounds and the inability to characterize bound phenolics or flavonoids that are isomeric.The review provides a systematic review of advanced analytical techniques used in selected fruit crops such as citrus, Amazonian fruits (mamey apple, camapu, uxi), unripe mangoes and grapes, sumac (Rhuscoriaria), Rhodomyrtustomentosa, and green calyx plum.UHPLC-Q-Orbitrap HRMS and LC-ESI-QTOF-MS/MS allowed the identification of 293 metabolites, such as gallotannins, proanthocyanidins, flavanones, bound phenolics, etc.Definitive structural elucidation was achieved by nuclear magnetic resonance (NMR) spectroscopy and electron paramagnetic resonance (EPR) showed radical-scavenging kinectic.Sumac had the highest antioxidant activity (DPPH IC₅₀ = 5.8 µg/mL; ABTS = 1245.8 µmol TE/g DW), which was followed by unripe mango and grape.Through principal component analysis (PCA) and partial least squares (PLS) regression, it was found that galloyl number had a strong correlation with ABTS values (R² = 0.96) and bound phenolics accounted for more than 45% of total activity in Rhodomyrtustomentosa.High-throughput screening was achieved by 94% of variance in antioxidant capacity explained by a predictive model using UHPLC-HRMS fingerprints.These sophisticated HPs are able to break the bottlenecks of existing platforms and offer mechanistic insights to help inform selection of fruit crops for functional food development.
Source: Oriental Journal Of Chemistry
DOI: 10.13005/ojc/420432
Language: English
Subfield: Biochemistry (medical)
Field: Medicine
Domain: Health Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W7204992575