Year: 2026
Type: preprint
Abstract: Goose astrovirus (GAstV) is a major pathogen associated with gout in goslings. Currently, no effective therapeutics or vaccines are available against GAstV infection, and disease control primarily relies on strict biosecurity measures. Therefore, the development of rapid and reliable diagnostic methods is critical for clinical management and reducing the impact of the virus. In this study, an indirect enzyme-linked immunosorbent assay (ELISA) for the detection of GAstV was developed. The N-terminal region (amino acids 130–401) of the GAstV capsid protein was cloned into the prokaryotic expression vector pET-32a(+) and expressed in Escherichia coli BL21 cells. The recombinant protein, designated GAstV-Cap-N, was purified and used as the coating antigen in the ELISA. Following systematic optimization of key parameters, the established indirect ELISA exhibited high specificity, sensitivity, and reproducibility. Comparative analysis with Western blot demonstrated a concordance rate of 87.5%. The assay was subsequently applied to test 228 clinical serum samples from geese, revealing an overall seropositivity rate of 38.19%. These findings indicate that the developed indirect ELISA provides a robust and reliable tool for clinical diagnosis and epidemiological surveillance of GAstV.
Source: Research Square
Institution: Guangxi University
Language: English
Subfield: Animal Science and Zoology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7204996878