Year: 2025
Type: conference-paper
Abstract: Antibiotics are crucial for medical treatment in both humans and animals, however, their overuse and widespread application raise significant health and environmental concerns. Among these, fluoroquinolones, a specific class of antibiotics, have become increasingly common in the environment, prompting worries due to their persistent nature and rising usage. In this study, we utilized the white-rot fungus Coriolopsis gallica, known for its abilityto effectively degrade the environmentally persistent fluoroquinolone, levofloxacin. We investigated the biotransformation of levofloxacin using the secretome of C. gallica and the purified laccase secreted by the same fungus. Various physicochemical factors were tested, including concentrations of 1-hydroxybenzotriazole (HBT), enzyme and antibiotic concentrations, as well as pH and temperature conditions for the biotransformation process. We compared the efficacy of free and immobilized secretome proteins from C. gallica and analyzed the resulting reaction products for residual activity against E. coli through growth inhibition tests. High-Performance Liquid Chromatography (HPLC) analysis was also conducted. The results indicated that the free secretome achieved a maximum removal efficiency of 50 mg L−1 of levofloxacin in the presence of 2.5 mM HBT. In contrast, the immobilized secretome was only able to degrade 10 mg L−1 of levofloxacin under the same mediator concentration, although it offered the advantage of reusability. These findings confirm the role of laccase in the biotransformation of levofloxacin. Additionally, we characterized the recombinant DyP1 produced by C. gallica and assessed its potential for biotransforming levofloxacin and six other fluoroquinolone antibiotics.
Source: HAL AMU
Language: English
Subfield: Pollution
Field: Environmental Science
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W7205296192