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Anxiolytic and Anticonvulsant Effects of Moringa Isothiocyanate-1 (MIC-1) in Adult Zebrafish

Year: 2026

Type: article

Abstract: The ‘miracle tree’ Moringa oleifera from the Moringaceae family is recognized for its rich nutritional value and therapeutic properties. This study aimed to evaluate the phytopharmaceutical compound Moringa isothiocyanate-1 (MIC-1) isolated from hydroalcoholic extract of M. oleifera seeds for the treatment of seizures and anxiety in adult zebrafish. MIC-1 (up to 0.5 mg/mL) did not show any acute toxic effects over a 96-h period. Moreover, MIC-1 (0.005 and 0.05 mg/mL) delayed the onset of seizures induced by pentylenetetrazol, indicating anticonvulsant activity. The anticonvulsant effects of MIC-1 (0.05 mg/mL) were reversed by flumazenil, suggesting a mechanism involving the GABAA receptor. Furthermore, MIC-1 (0.05 mg/mL) has anxiolytic effects, with involvement of the serotonergic and GABAergic systems. In silico assays confirmed the interaction of MIC-1 with the GABAA, 5-HT1B, 5-HT2A/C, and 5-HT3A/B receptors. Additionally, MIC-1 was effective in treating anxiety related to alcohol withdrawal in zebrafish. These findings suggest the pharmacological potential of MIC-1 for the development of new treatments for seizures and anxiety.

Source: Biology

DOI: 10.3390/biology15171523