Fetal Vertebral Anomalies Induced by Calcium Channel Blockers in Rats
Year: 2021
Type: article
Abstract: The present study aimed at examining skeletal anomalies of fetuses induced by verapamil, calcium channel blocker, in rats, and comparing the skeletal morphological features and locations to those by another calcium channel blocker of diltiazem. Verapamil at 30 mg/kg or diltiazem at 90 mg/kg were given by a single intraperitoneal injection to pregnant rats on gestation day 11, and cesarean section was conducted and fetuses were obtained on gestation day 21. Fetal skeletons were stained with Alizarin red S and Alcian blue, and examined for the skeletal anomalies. Verapamil and diltiazem increased the incidences of fetuses with skeletal anomalies in the thoracic, lumbar, sacral or caudal vertebrae and the types of anomalies included absent, fused, misshapen and/or split shapes. The affected vertebrae by verapamil or diltiazem were observed from late number of thoracic vertebrae to early number of caudal vertebrae. These results indicate that verapamil-induced vertebral skeletal anomalies were similar in morphological features and locations to those by diltiazem, suggesting that the fetal vertebral anomalies might be common by basic pharmacological properties of verapamil and diltiazem in rats. Key Words: Calcium channel blocker, Diltiazem, Fetus, Rats, Verapamil, Vertebral anomaly
Authors: 坂, 芳樹, 堀本, 政夫, BAN, Yoshiki
Language: English
Field: Medicine
Domain: Health Sciences
Open Access status: green
OpenAlex ID: W7208097266