Characterization and antibacterial potential of aqueous and ethanolic extracts of Combretum collinum Fresen (Combretaceae) trunk bark against extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae strains
Year: 2026
Type: article
Abstract: The rise of bacterial resistance is shaking the foundations of conventional antibiotics, prompting medical research to explore new therapeutic avenues. It is within this context that this study was conducted, dedicated to exploring the antibacterial potential of aqueous and ethanolic stem extracts of Combretum collinum Fresen (Combretaceae). The evaluation targeted a reference strain and 10 clinical isolates of extended-spectrum beta-lactamase (ESBL)-producing Enterobacteriaceae, using a combination of well diffusion on agar and macrodilution in liquid medium. Furthermore, thin-layer chromatography (TLC) analysis was performed to map the active secondary metabolites. The results reveal widespread susceptibility of the strains, although the intensity varied depending on the specific extract. At a concentration of 50 mg/ml, the ethanolic fraction proved particularly effective against the opportunistic pathogen Shigella sp. 55C, inducing a zone of inhibition of 22 ± 4.1 mm. Quantitatively, this ethanolic extract exhibited a purely bactericidal profile across the entire panel (MICs ranging from 0.78 to 6.24 mg/ml), while the aqueous extract eradicated 66% of the tested organisms. Phytochemical screening revealed a high content of tannins, flavonoids, polyterpenes, anthocyanins, and polyphenols. These bioactive molecules support the observed efficacy against enterobacteria and provide a solid scientific basis for the empirical use of Combretum collinum in traditional medicine.
Source: Acta Botanica Plantae
Authors: SANOGO Yacouba, BAMBA Moussa, KONE Dramane
Language: English
Subfield: Food Science
Domain: Life Sciences
Open Access status: bronze
OpenAlex ID: W7212224560