Simultaneous Estimation of Dapagliflozin and Bisoprolol Fumarate in Pharmaceutical Formulation by UV Spectroscopic Method Using Simultaneous Equation Method (Vierordt's Method)
Year: 2026
Type: article
Abstract: A simple, accurate and reliable UV-visible spectrophotometric method was developed and validated for the simultaneous estimation of Dapagliflozin (DAPA) and Bisoprolol fumarate (BISO) in bulk drug samples and pharmaceutical dosage forms. Methanol was selected as the solvent based on solubility studies of both drugs. Dapagliflozin showed maximum absorbance at 222nm, While Bisoprolol Fumarate showed maximum absorbance at 270nm. The linearity range for both drugs was found to be 2-10μg/ml with correlation coefficients of 0.999 Dapagliflozin and 0.9947 for Bisoprolol fumarate, indicating excellent linearity. Various analytical validation parameters i.e., Accuracy, Precision, LOD and LOQ were calculated using standards. In order to check the performance assay was carried for the marketed formulation was found to be 98.52% and 97.98% respectively, LOD and LOQ for DAPA were found to be 0.07947 and 0.24082μg/ml and for BISO, it was found to be 0.34494 and 1.04526μg/ml. The results % Recovery for DAPA and BISO were observed in the range of 98.58 - 100.94% and 100.87 – 101.08%. The precision study was found to be less than 2% RSD for both drugs. The developed method can be used for the simultaneous estimation of DAPA and BISO from tablet formulation.
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: hybrid
OpenAlex ID: W7213790695