Middle Cerebral Artery Occlusion Model of Stroke in Rodents: a Stepby-Step Approach
Year: 2023
Type: article
Abstract: Stroke is one of the leading causes of morbidity and mortality in developed countries and an immenseamount of medical care resources are devoted to combat the poststroke debilitating consequences. The keyto develop effective and clinically applicable treatment methodologies is a better understanding of the pathophysiology of the disease, including the root causes and targets for pharmacology. Developing these foundations requires the use of standard animal models that mimic the physicochemical process of the diseasesthat can reliably replicate results in order to test and fine-tune therapeutic modalities. Middle cerebral arteryocclusion (MCAO), endothelin-1-induced ischemic stroke, photothrombosis, devascularization, embolization, and spontaneous infarction using hemorrhage are some examples of different animal models. Reliability of MCAO has been proved and due to the ability to induce reperfusion similar to tissue plasminogenactivator (tPA) therapy, this model is widely used in preclinical studies.Here, we describe a detailed methodology on how to develop MCAO stroke in rodents using intra-arterialinsertion of filament to occlude the middle cerebral artery. This approach allows for the study of a widearray of basic pathophysiology mechanisms, regenerative medicine and rehabilitation therapy.
Language: English
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7214820243