Enhanced Bioethanol Production by Saccharomyces Cerevisiae: Improving Saccharification Efficiency, Fermentation Performance, and Tolerance to Acidic And Osmotic Stress
Year: 2026
Type: article
Abstract: The growing demand for renewable energy has driven the development of innovative methods to enhance energy production from sustainable sources. Ethanol production from molasses is a well-established approach that contributes significantly to this effort. In this study, the fermentation process was optimized to maximize ethanol yield by investigating the effect of various operational parameters using the enzyme Zymo Faan as an additive. The parameters examined in the study included aeration rate (ranging from 50 to 300 ml/min), agitation speed (100 to 500 rpm), pH (4.0 to 7.0), and temperature (25°C to 45°C). Maximum ethanol production of approximately 86.2 g/l was achieved under the optimized conditions of pH 5.5, temperature 35°C, agitation speed 300 rpm, and an aeration rate of 200 ml/min, using an enzyme dose of 5.0 g/l. Under these conditions, the ethanol yield reached 0.43 g ethanol/g substrate, with a productivity of 1.79 g/l/h over a fermentation period of 48 hours. The maximum ethanol concentration achieved with the addition of 5 g/l of enzyme was 86 g/l, compared to 64 g/l without the enzyme. This represents a 34.4% increase in ethanol production The results demonstrate that the addition of Zymo Faan significantly enhances ethanol productivity compared to fermentation without the enzyme. Future research could focus on studying the kinetics of Zymo Faan to enable further process optimization and scaling.
Institution: Dawood University of Engineering and Technology
Language: English
Subfield: Biomedical Engineering
Field: Engineering
Domain: Physical Sciences
Open Access status: hybrid
OpenAlex ID: W7214902160