Year: 2026
Type: article
Abstract: The aim of the study was a toxicological assessment of the cumulative properties of a new pharmaceutical composition "A-14" (ointment), synthesized under the guidance of Professor I.V. Galkina. The active pharmaceutical ingredient of the composition is azomethine, with dioxomethyltetrahydropyrimidine and methyluracil used as excipients. The experiment was conducted on laborato-ry rats, randomized into test and control groups. The test group was subjected to a step dose escalation regimen: the starting dose was 580 mg/kg (1/10 of the maximum tolerated dose established in an acute experiment), with a subsequent 1.5-fold increase every 96 hours (4 days). Animals in the control group received an equivalent volume of distilled water. As a result of the study, it was found that repeated administration of the "A-14" composition at a cumulative dose of 4406 mg/kg resulted in 100% lethality in the test group. Pathomorphological analysis revealed a complex of pathological changes in deceased animals, including hyperemia of parenchymal organs, granular and fatty degeneration, as well as multiple hemorrhages. The total cumulative dose administered to the animals over the entire experimental period reached 74660 mg/kg. Based on the data obtained, the cumulative coefficient (Kcum = LD₅₀ cumulative / LD₅₀ single) was calculated and amounted to 12.9, which, according to the classification of L.I. Medved, indicates a low cumulative potential of the studied pharmaceutical composition.
Language: English
Subfield: Immunology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7214938183