การพัฒนาวิธีตรวจหาสารพันธุกรรมของไวรัสคางทูมในตัวอย่างส่งตรวจด้วยวิธี Reverse Transcription-Polymerase Chain Reaction (RT-PCR)
Year: 2014
Type: article
Abstract: Infection with mumps virus results in swelling of salivary glands and further complications such as orchitis, meningitis or abortion within the first-trimester of pregnancy may occur. We, therefore, developed an RT-PCR method for detection of mumps genetic materials in clinical specimens. It was found that the limit of detection for our in-house RT-PCR method is 10 copies or 40 ag (10-18 g) and there is no cross – reactivity with measles virus, the other virus within the same family. It was demonstrated that the developed RT-PCR method is able to detect the genetic materials of mumps virus in saliva, throat swab, buccal swab, CSF, semen and mumps-infected cell lysate. The in-house RT-PCR technic showed 100% correlation with the gold standard method, i.e., viral isolation. The result from our study indicated that the developed RT-PCR method is useful for laboratory diagnosis as well as genetic characterization of mumps virus for the outbreak investigation in Thailand.
Language: Thai
Topic: Virology and Viral Diseases
Subfield: Epidemiology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7216697358