Developing Methods for Selecting Common Bean (Phaseolus vulgaris L.) Varieties with High Seed Protein Levels
Year: 2025
Type: dissertation
Abstract: Phaseolus vulgaris are legumes that are major sources of dietary protein for humans, worldwide. To ensure future food security for increasing human populations there is a need to develop methods to rapidly measure and select for protein contents in beans. In this study, a near infrared (NIR) calibration curve for total protein content in common bean was created with lab and spectrometer data from seed samples of 146 lines from 8 market classes. This curve allows for the rapid and non-destructive determination of total protein in bean seeds. A genotype by sequencing (GBS) study found 16 single nucleotide polymorphisms (SNPs) across all chromosomes associated with total protein in a 212-genotype collection of common beans through 3 genome wide association studies (GWAS); one from Elora, ON, one from Woodstock, ON and one combined analysis These SNPs can be used as selection criteria for lines with increased total protein seed content.
Author: Gallo, Holly
Institution: University of Guelph
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
SDG: Zero hunger
Open Access status: green
OpenAlex ID: W7217634767