ISOLATION OF TRACHELANTHAMINE N-OXIDE ALKALOID FROM Heliotropium lasiocarpum
Year: 2026
Type: conference-paper
Abstract: Heliotropium lasiocarpum is an annual poisonous herb belonging to the family Boraginaceae. Previous phytochemical studies have shown that the stems and leaves contain up to 0.4% alkaloids, whereas the seeds contain approximately 1.0% alkaloids. The aerial parts of H. lasiocarpum were collected during the flowering stage in 2022 from the foothill region of Yangiyer District, Syrdarya Region, Uzbekistan. The air-dried and powdered plant material was extracted with 80% methanol using a multifunctional ultrasonic extraction system. The extraction was carried out seven times at 30°C, with each extraction lasting 30 min. The crude alkaloid extract was further fractionated according to solvent polarity into CHCl₃-, EtOAc-, and n-BuOH-soluble alkaloid fractions. The ethyl acetate (EtOAc) alkaloid fraction was subjected to silica gel column chromatography (SiO₂, 60–100 мm, sample-to-sorbent ratio 1:30) to isolate individual alkaloids. Elution was performed with a CHCl₃–MeOH solvent system using a gradient from 200:1 to 1:1. As a result, compound 1 was isolated (19.0 mg, corresponding to 0.0019% of the dry plant material). The compound possessed the molecular formula C₁₅H₂₇NO₅ and exhibited a melting point of 165–167°C. Its ESI-MS spectrum (positive ion mode) showed a protonated molecular ion peak at m/z 300.4681 [M+H]⁺, which is consistent with the molecular formula C₁₅H₂₈NO₅ (calculated m/z 300.4015).
Authors: H.N. Olimova, U.Kh. Kurbanov, N.I. Mukarramov
Language: English
Subfield: Organic Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: green
OpenAlex ID: W7218397358