Phenotypic and genotypic insights into a halotolerant bacterium with antimicrobial potential from El Golea Lake
Year: 2024
Type: article
Abstract: Introduction: The antimicrobial potential of a halotolerant bacterial strain, LMB3982, isolated from thehypersaline Lake El Golea in Algeria at 25% NaCl (w/v), was investigated. The study aimed to evaluate theantimicrobial activity of this strain and to characterize its phenotypic and genotypic properties, with a focus onits bioactive compounds and environmental adaptability.Methods: The strain was subjected to primary and secondary screenings for antimicrobial activity against apanel of test microorganisms. The influence of growth medium and incubation time on the production ofantimicrobial metabolites was assessed. Various organic solvents were used to extract antimicrobial compounds,which were then evaluated for their efficacy. Thin-layer chromatography (TLC) was performed on chloroformicextracts using three solvent systems, and chemical properties were analyzed using infrared (IR) s pectroscopy.Additionally, a taxonomic study based on phenotypic and genotypic identification, including 16S rRNA genesequencing, was conducted.Results: Strain LMB3982 demonstrated significant antimicrobial activity against most test microorganisms, withmaximum activity observed after 72 hours of culture on nutrient agar. Chloroform was identified as the mosteffective solvent for extracting bioactive compounds. TLC analysis revealed the presence of two types ofmolecules: amines and phenols. IR spectros copy confirmed these findings, showing characteristic peakscorresponding to these compounds. Biochemical tests indicated that the strain could produce various enzymes,utilize multiple sugars, and grow under a wide range of conditions: NaCl concentrations of 0–25%, pH levels of5–11, and temperatures of 15–37°C. Phylogenetic analysis of the 16S rRNA gene sequence positioned strainLMB3982 as a novel taxon within the Bacillales order, showing 96% similarity to Bacillus pseudofirmus and93.3% to Bacillus haloduransConclusion:. These findings highlight the potential of strain LMB3982 for biotechnological applications,particularly in the production of novel antimicrobial compounds and enzymes adapted to extreme conditions.
Authors: Fatima Nas, Nihel Klouche Khelil
Language: English
Subfield: Applied Microbiology and Biotechnology
Field: Immunology and Microbiology
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W7219933401