การต้านอนุมูลอิสระ ปริมาณรวมของสารประกอบฟีนอลิก และสมบัติการสมานแผลของสารสกัดจากส้มโอพันธุ์ขาวใหญ่
Year: 2024
Type: article
Abstract: This research investigated the biological properties of different parts of Pomelo (Citrus maxima (Burm.f.) Merr.) extract involving antioxidant activity, total phenolic content, and wound healing activity. The extraction process employed the maceration in hexane and methanol, utilizing various parts of pomelo, namely flavedo (FL), albedo (AL), segmented membrane (SM), and juice sac (JS). The results indicated that the methanolic extract of JS demonstrated antioxidant activity with an IC50 of 1.021 ± 0.031 mg/mL by DPPH scavenging method, while methanolic extract of AL exhibited an IC50 of 0.126 ± 0.004 mg/mL by ABTS scavenging method. The highest total phenolic content was determined in methanolic extract of SM with the value of 3.196 ± 0.022 mg GAE g-1 dry material. Moreover, the cytotoxicity assay conducted on HaCaT cells using MTT revealed that extract from both hexane and methanol did not exhibit cytotoxicity at concentrations of 0.5 and 1 μg/mL respectively. Interestingly, at non-toxic dose, hexane and methanolic extracts of AL and methanolic extract of FL showed a significant reduction in wound area compared with control (p < 0.05). In conclusion, extracts from C. maxima (Burm.f.) Merr. exhibit both antioxidant and wound-healing activity. This study revealed significant promises for advancing research and development in wound healing products for patients dealing with chronic wound issues. Furthermore, it explores an alternative utilization of agricultural waste, presenting potential avenues for its application.
Institution: Huachiew Chalermprakiet University
Language: Thai
Subfield: Biochemistry (medical)
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
OpenAlex ID: W7220114746