Karakteristik morfologis dan aktivitas antibakteri bakteri endofit asal akar Smilax rotundifolia L. terhadap Staphylococcus aureus dan Pseudomonas aeruginosa: Morphological characteristics and antibacterial activity of endophytic bacteria from the roots of Smilax rotundifolia L. against Staphylococcus aureus and Pseudomonas aeruginosa
Year: 2026
Type: article
Abstract: ABSTRACT: Endophytic bacteria are microorganisms that colonize plant tissues without harming their host. Most plant endophytic bacteria in plants can produce a range of bioactive compounds with potency comparable to that of their hosts, as exemplified by Smilax rotundifolia, which is recognized for its antibacterial properties. This study aims to characterize the colony and cell morphology of endophytic bacteria isolated from the roots of S. rotundifolia and to evaluate their antibacterial activity against Staphylococcus aureus and Pseudomonas aeruginosa. Characterization was performed macroscopically on nutrient agar medium and microscopically using Gram staining. Antibacterial activity was assessed through the disc diffusion method with three replicates per treatment. Data analysis was conducted descriptively and with analysis of variance (ANOVA), followed by Duncan’s Multiple Range Test (DMRT) at a significance level of 5%. Eight endophytic bacteria isolated from S. rotundifolia roots had white colonies with diverse shapes, margins, and elevations. All eight isolates were identified as Gram-negative and bacillus-shaped bacteria. Supernatants from isolates ASR 1 and ASR 4 demonstrated moderate inhibitory activity against S. aureus. The best antibacterial activity among the eight ASR isolates was shown by the culture supernatant of isolates ASR 1 and ASR 4, which exhibited inhibitory activity against S. aureus in the weak category. The diameter of the inhibition zones of ASR1 and ASR4 supernatants against of S. aureus were 2,7+0,6 and 2,9+0,4 mm, respectively. The antibacterial activity of endophytic bacterial isolates ASR 1 and ASR 4 from S. rotundifolia roots shows potential as natural antibacterials. However, their inhibitory activity is currently classified as moderate, necessitating further optimization and purification of antibacterial compounds in future research.
Source: Cassowary
Institution: Universitas Papua
Language: English
Subfield: Plant Science
Domain: Life Sciences
Open Access status: diamond
OpenAlex ID: W7220378286