High Performance Thin Layer Chromatography (HPTLC) Analysis of Anti-hypertensive Combination Therapy in Pharmaceutical Dosage form
Year: 2026
Type: article
Abstract: A simple, selective, rapid and sensitive high performance thin-layer chromatography (HPTLC)-densitometric method was developed and validated for the simultaneous determination of Amlodipine besylate, Telmisartan and Indapamide in bulk and pharmaceutical dosage form. Chromatographic separation achieved on precoated silica gel 60 F 254 plates using chloroform: toluene: ethyl acetate: methanol: TEA(4:3:1.5:1.5:0.1, v/v) as the mobile phase with densitometric detection at 240 nm. The analytes showed satisfactory separation with Rf values of 0.21± 0.0004, 0.53 ±0.002 and 0.64 ± 0.003 for AMLO, TELMI and INDA, respectively. The method exhibited excellent linearity over the ranges of 250-1250 ng/band for AMLO, 2000-10000 ng/band for TELMI and 125-625 ng/band for INDA. Accuracy studies demonstrated recoveries within 98-102 %, while precision studies showed %RSD values below 2 %. The method remained robust following deliberate variation in mobile phase composition, migration distance and chamber saturation time. Application of the method to the pharmaceutical formulation demonstrated assay values within the acceptable range, with no interference from excipients. Compared with convention HPLC methods, the proposed HPTLC procedure offers rapid analysis, lower solvent consumption, operational simplicity and cost effectiveness making it suitable for routine quality control of AMLO, TELMI and INDA in pharmaceutical formulations.
Institution: Siksha O Anusandhan University
Language: English
Subfield: Analytical Chemistry
Field: Chemistry
Domain: Physical Sciences
Open Access status: bronze
OpenAlex ID: W7222787285