Galactooligosaccharides Suppress the Development of Biofilm in the Presence of Blood Polymorphonuclear Cells of Non-Small Cell Lung Cancer (NSCLC) Patients: A Pilot Study.
Year: 2025
Type: article
Abstract: Background: Bacterial biofilms were investigated in the presence or absence of PMNs isolated from the blood of 12 NSCLC patients and 3 healthy controls (HC), with the inclusion of GOS. These PMNs were incubated with GFP-SA or GFP-PA biofilms and exposed to various concentrations of GOS (2.5%, 5%, 10%, 15%, and 20%) for 24 hours in a microplate setting. The progress of bacterial biofilm was quantified using the crystal violet assay. Results: GOS itself, with bacteria at high concentrations (10%, 15%, and 20%), suppressed biofilm formation. PMNs of NSCLC patients show higher biofilm formation than in the absence of PMNs. Conclusion: Based on our findings, PMNs in NSCLC patients may disrupt the natural balance of bacteria in the lungs, potentially leading to an overgrowth of biofilm-forming bacteria. GOS are known to influence immune function and may improve the ability of PMNs to fight infections. Consequently, it is proposed that GOS could enhance the ability of PMNs to phagocytose bacterial biofilms and counteract the negative effects of cancerous PMNs on biofilm formation. However, further research is needed to confirm whether GOS can effectively manage bacterial biofilms in lung cancer patients.
Source: PubMed Central
Language: English
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
OpenAlex ID: W7222906863