Phytochemical Profiling and Renoprotective Potential of Crude Ethanolic Extract and N-hexane Fraction of Lagerstroemia Indica on Mouse Model of Lipopolysaccharide-induced Kidney Injury
Year: 2026
Type: article
Abstract: Lagerstroemia indica L. (Lythraceae) is an ornamental plant traditionally used for treating various diseases, but its phytochemical composition and nephroprotective activity are undeveloped. This study aimed to explore the phytochemical ingredients and nephroprotective effect of the crude ethanolic extract and n-hexane fraction of Lagerstroemia indica against LPS-induced acute kidney injury (AKI) in mice. The plant was sequentially extracted with n-hexane followed by 85% ethanol using Soxhlet method. The n-hexane fraction was qualitatively analyzed using gas chromatography-mass spectroscopy (GC-MS). Forty male albino mice were randomly allocated into four groups (n=10): normal control, LPS group, n-hexane fraction (100 mg/kg) group, and crude ethanolic extract (100 mg/kg) group. Treatment was administered orally for five consecutive days before induction of AKI by intraperitoneal injection of LPS (10 mg/kg). Molecular analysis confirmed the identity of the investigated plant as L. indica with 99% sequence similarity to the reference chloroplast genome. GC/MS analysis of the n-hexane fraction identified twenty-one compounds. LPS administration significantly increased serum urea and creatinine concentrations, upregulated TNF-α, NF-kB, and Caspase-3 expression, altered KIM-1 expression, and reduced Nrf2 expression. Pretreatment with both crude ethanolic extract and n-hexane fraction significantly improved renal function and attenuated inflammatory, apoptotic, and histopathological alterations. However, the crude ethanolic extract produced greater nephroprotective effects than the n-hexane fraction. In conclusion, Iraqi Lagerstroemia indica possesses profound nephroprotective action against LPS-induced acute kidney damage mediated via attenuation of inflammation and apoptosis together with the enhancement of antioxidant defense.
Source: Acta Innovations
Institutions: University of Baghdad, Al-Esraa University
Language: English
Topic: Acute Kidney Injury Research
Subfield: Nephrology
Field: Medicine
Domain: Health Sciences
Open Access status: diamond
Funder: University of Baghdad
OpenAlex ID: W7225814985
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