Methods for the quantitative study of the aerobic and anaerobic intestinal bacterial flora of man.
Year: 1968
Type: article
Abstract: The methods employed for the quantitative isolation of stool bacteria in man have not been standardized. Schaedler, Dubos, and Costello have outlined in detail their methods for isolation of aerobic and anaerobic bacteria in specimens obtained from mice. ' However, in vitro growth factors for the isolation of the bacterial flora of mice are not applicable at all times to man. While undertaking a study of variations of intestinal bacteria in human disease states, it became apparent that considerable effort was required to establish and standardize reproducible bacteriologic techniques. The present report is a detailed account of the methods we found most satisfactory along with data obtained in normal subjects. METHODS Stool collection. Patients were given a sterile disposable petri dish with an applicator stick and asked to deposit a small amount of freshly passed fecal material in the dish. The specimen was cultured within 15 to 30 minutes. (Present data indicate that cold or room temperature storage does not preserve the quantitative relationships of
Source: PubMed Central
Authors: M H Floch, W Gershengoren, L R Freedman
Language: English
Topic: Gut microbiota and health
Subfield: Molecular Biology
Domain: Life Sciences
Open Access status: green
Funders: U.S. Army, U.S. Public Health Service
OpenAlex ID: W74428258