Development of the SCAR markers linked to the Sh gene in buckwheat
Year: 1999
Type: article
Abstract: Three RAPD markers, OPBI41250, OPP81000 and OPQ7 goo, tightly linked to Sh gene were cloned and char-acterized. Nucleotide sequence information, obtained from each end of the three linked RAPD markers, was used to design 21-mer oligonucleotide primers for PCR amplification of the respective SCARs. Two of these SCAR primers, SCB141250 and SCP8 1000, amplified a single fragment in Fagopyrum homotropicum but was absent in common buckwheat, F. esculentum. In one case, SCQ7goo, showed different size PCR fragments in common buckwheat than in F. homotropicum. This co-dominant marker is useful for differentiating hetero-zygosity from both types of homozygote.
Authors: Jotaro Aii, Mio Nagano, Woo Sun‐Hee, Colin Campbell, Tetsuya Adachi
Language: English
Subfield: Genetics
Domain: Life Sciences
SDG: Zero hunger
Open Access status: diamond
OpenAlex ID: W74896492